Identification of a short rDNA spacer sequence highly specific of a tomato line containing Tm-1 gene introgressed from Lycopersicon hirsutum
Identifieur interne : 000277 ( France/Analysis ); précédent : 000276; suivant : 000278Identification of a short rDNA spacer sequence highly specific of a tomato line containing Tm-1 gene introgressed from Lycopersicon hirsutum
Auteurs : H. Levesque [France] ; F. Vedel [France] ; C. Mathieu [France] ; L. De Courcel [France]Source :
- Theoretical and Applied Genetics [ 0040-5752 ] ; 1990-11-01.
Abstract
Summary: We studied rDNA restriction fragment length polymorphism between two tomato lines used for F1 hybrid seed production: line A, containing the Tm-1 gene responsible for tobacco mosaic virus tolerance introgressed from the wild species Lycopersicon hirsutum, and line B, a tobacco mosaic virus sensitive line. Hybridization patterns led to distinct rDNA maps with two size classes, 10.4 and 10.7 kb, in line A and a single, 8.9-kb class in line B. Size differences were located in the intergenie sequence (IGS). A highly specific 54-bp TaqI fragment was cloned from the line A IGS and used in dot blot experiments to probe total DNA from line A, line B, and their F1 hybrid. It proved capable of discriminating B from A and the hybrid. This probe could thus serve to screen inbreds in commercial seed lots where line A is used as male. This fragment showed 80–90% sequence homology with the 53-bp subrepeats previously characterized in a region of the tomato IGS close to the 25S rRNA gene. Preliminary comparison of rDNA in line A and several wild related species indicated that the L. hirsutum H2 genotype was the closest to line A. rDNA variations between line A and this wild genotype could be explained by recombination during the introgression process involving numerous backcrosses or by an important intraspecific polymorphism. Our results strongly suggest that Tm-1 and the rDNA were introgressed together into tomato from L. hirsutum through linkage drag.
Url:
DOI: 10.1007/BF00224218
Affiliations:
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Genetics</title><idno type="ISSN">0040-5752</idno><idno type="eISSN">1432-2242</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1990-11-01">1990-11-01</date><biblScope unit="volume">80</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="602">602</biblScope><biblScope unit="page" to="608">608</biblScope></imprint><idno type="ISSN">0040-5752</idno></series><idno type="istex">CDD2228DDFF465A87AB7F76006C2DB18F0D0B7EF</idno><idno type="DOI">10.1007/BF00224218</idno><idno type="ArticleID">BF00224218</idno><idno type="ArticleID">Art4</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0040-5752</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: We studied rDNA restriction fragment length polymorphism between two tomato lines used for F1 hybrid seed production: line A, containing the Tm-1 gene responsible for tobacco mosaic virus tolerance introgressed from the wild species Lycopersicon hirsutum, and line B, a tobacco mosaic virus sensitive line. Hybridization patterns led to distinct rDNA maps with two size classes, 10.4 and 10.7 kb, in line A and a single, 8.9-kb class in line B. Size differences were located in the intergenie sequence (IGS). A highly specific 54-bp TaqI fragment was cloned from the line A IGS and used in dot blot experiments to probe total DNA from line A, line B, and their F1 hybrid. It proved capable of discriminating B from A and the hybrid. This probe could thus serve to screen inbreds in commercial seed lots where line A is used as male. This fragment showed 80–90% sequence homology with the 53-bp subrepeats previously characterized in a region of the tomato IGS close to the 25S rRNA gene. Preliminary comparison of rDNA in line A and several wild related species indicated that the L. hirsutum H2 genotype was the closest to line A. rDNA variations between line A and this wild genotype could be explained by recombination during the introgression process involving numerous backcrosses or by an important intraspecific polymorphism. Our results strongly suggest that Tm-1 and the rDNA were introgressed together into tomato from L. hirsutum through linkage drag.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Brétigny sur Orge</li><li>Orsay</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Levesque, H" sort="Levesque, H" uniqKey="Levesque H" first="H." last="Levesque">H. Levesque</name></region><name sortKey="De Courcel, L" sort="De Courcel, L" uniqKey="De Courcel L" first="L." last="De Courcel">L. De Courcel</name><name sortKey="Mathieu, C" sort="Mathieu, C" uniqKey="Mathieu C" first="C." last="Mathieu">C. Mathieu</name><name sortKey="Vedel, F" sort="Vedel, F" uniqKey="Vedel F" first="F." last="Vedel">F. Vedel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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